Txhawm rau soj ntsuam cov nyhuv ntawm cov qog nqaij hlav I ntawm Agkistrodon acutus venom (AAVC-1) ntawm kev tsiv teb tsaws ntawm tib neeg cov hlab ntsha endothelial hlwb (HUVECs), thiab tshawb nrhiav qhov ua tau ntawm AAVC - Ⅰ inhibiting angiogenesis.Cov txheej txheem: HUVECs tau coj los rau hauv vitro nrog AAVC - Ⅰ (0, 20, 40, 80 μ G / ml) kho cov hlwb raug incubated rau 24 teev.Scratch test thiab chemotactic chamber test tau siv los soj ntsuam cov txiaj ntsig ntawm AAVC - Ⅰ ntawm endothelial cell migration activation;RT-PCR thiab Western blot tau siv los kuaj xyuas qhov hloov pauv ntawm mRNA thiab qib protein ntawm P-selectin thiab intercellular adhesion factor (ICAM-1) ua ntej thiab tom qab kev kho tshuaj.Cov txiaj ntsig: Piv nrog HU VECs hauv cov pab pawg ib txwm muaj, qhov peev xwm tsiv teb tsaws ntawm cov hlwb hauv AAVC - Ⅰ pawg concentration tau txo qis rau qib sib txawv, thiab kev qhia ntawm P-selectin thiab ICAM-1 mRNA poob qis.Cov ntsiab lus: AAVC - Ⅰ tuaj yeem cuam tshuam kev tsiv teb tsaws ntawm cov hlwb endothelial los ntawm kev tswj hwm mRNA thiab protein ntau ntawm P-selectin thiab ICAM-1
Post lub sij hawm: Dec-07-2022