IgY antibody tiv thaiv cov motif ntau ntawm metalloproteinases los ntawm Agkistrodon acutus venom tau npaj thiab nws lub luag haujlwm hauv kev ua si nab venom tau kawm [Txoj Kev] Los ntawm kev tshuaj xyuas cov kab ke ntawm ntau yam metalloproteinases los ntawm Agkistrodon acutus venom, peb tsim thiab ua kom muaj kev txuag antigenic PT2 peptides. , uas muaj feem xyuam nrog lawv cov enzyme kev ua haujlwm;Cov hens tau txhaj tshuaj nrog cov sib txuas sib txuas KLH-PT1 thiab KLH-PT2 kom tau txais cov tshuaj tiv thaiv IgY.Lub titer ntawm IgY thiab tus ntoo khaub lig cov yam ntxwv nrog cov venom ntawm Agkistrodon acutus thiab Agkistrodon brevius tau kawm ua ntej los ntawm ELISA thiab Western blot;Thaum kawg, kev ua haujlwm nruab nrab ntawm IgY tau soj ntsuam los ntawm kev ntsuas tshuaj tiv thaiv subcutaneous hemorrhage hauv cov nas [Tshaj Tawm] ELISA thiab Western blot pom tias anti KLH-PT1 thiab anti Agkistrodon acutus venom IgY tuaj yeem cuam tshuam nrog Agkistrodon acutus venom thiab Agkistrodon brevius venom, tau muaj zog ntau dua li qub;Cov tshuaj tiv thaiv KLH-PT2 IgY tsis muaj qhov cuam tshuam tseem ceeb nrog Agkistrodon acutus thiab Agkistrodon brevius venoms hauv vitro, tab sis pom muaj zog tsis zoo rau kev ua kom muaj zog hauv cov tshuaj tiv thaiv los ntshav hauv vivo, thiab tau zoo dua rau yav dhau los ob [Xaiv] Cov tshuaj tiv thaiv IgY uas tuaj yeem ua tau. cross react nrog ntau yam ntshav ncig nab venoms tau npaj los ntawm kev tsim cov antigen peptide sib koom los ntawm metalloproteinases, uas nteg lub hauv paus rau kev npaj tshwj xeeb, npaum, tsis tshua muaj toxicity thiab broad-spectrum anti hemorrhagic nab venom antibody.
Post lub sij hawm: Dec-26-2022