Snake venom thrombin zoo li enzyme (TLE) yog ib qho serine protease ntawm trypsin tsev neeg, uas muaj kev txuag ntau dua nrog trypsin.Nws muaj arginine esterase kev ua, tuaj yeem ua ncaj qha rau fibrinogen, catalyze lub cleavage ntawm Arg2Gly peptide daim ntawv cog lus ntawm ib feem ntawm fibrinogen molecule, tso fibrinopeptide A (FPA) los yog B (FPB. Ob peb alkaline thrombins tau sib cais los ntawm cation txauv kem, ua ke nrog. gel filtration thiab affinity chromatography, Affinity chromatography yog dav siv vim nws cov kev sib cais siab thiab cov qauv zoo rov qab. Cov txheej txheem purification sib txawv yog siv, thiab cov khoom tau txais kuj txawv. Guo Chunteng li al. [6, 7] tau thrombin zoo li cov khoom siv P3 thiab P4 los ntawm cov venom ntawm Agkistrodon acutus hauv Fujian los ntawm kev siv cov txheej txheem purification sib txawv, kev tshawb fawb qhia tias ob leeg muaj thrombin kev ua si, tab sis EDTA ib feem inhibits thrombin kev ua ntawm cov khoom P4 thiab tag nrho inhibits coagulase kev ua ntawm tivthaiv P3. Cheebtsam P4 tsis muaj. kev ua haujlwm ntawm activating factor
Post lub sij hawm: Dec-23-2022